Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber.
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چکیده
منابع مشابه
Structure of a protein superfiber: spider dragline silk.
Spider major ampullate (dragline) silk is an extracellular fibrous protein with unique characteristics of strength and elasticity. The silk fiber has been proposed to consist of pseudocrystalline regions of antiparallel beta-sheet interspersed with elastic amorphous segments. The repetitive sequence of a fibroin protein from major ampullate silk of the spider Nephila clavipes was determined fro...
متن کاملAntheraea pernyi Silk Fiber: A Potential Resource for Artificially Biospinning Spider Dragline Silk
The outstanding properties of spider dragline silk are likely to be determined by a combination of the primary sequences and the secondary structure of the silk proteins. Antheraea pernyi silk has more similar sequences to spider dragline silk than the silk from its domestic counterpart, Bombyx mori. This makes it much potential as a resource for biospinning spider dragline silk. This paper fur...
متن کاملA two-dimensional spin-diffusion NMR study on the local structure of a water-soluble model peptide for Nephila clavipes dragline silk (MaSp1) before and after spinning
INTRODUCTION The dragline silk of the golden orb web spider Nephila clavipes has received significant attention because of its remarkable mechanical properties, which include toughness and high tensile strength.1,2 This silk contains two structural proteins designated as major ampullate spidroin 1 (MaSp1) and major ampullate spidroin 2 (MaSp2).3,4 The dominant MaSp1 can be described as AB block...
متن کاملHigh-Toughness Silk Produced by a Transgenic Silkworm Expressing Spider (Araneus ventricosus) Dragline Silk Protein
Spider dragline silk is a natural fiber that has excellent tensile properties; however, it is difficult to produce artificially as a long, strong fiber. Here, the spider (Araneus ventricosus) dragline protein gene was cloned and a transgenic silkworm was generated, that expressed the fusion protein of the fibroin heavy chain and spider dragline protein in cocoon silk. The spider silk protein co...
متن کاملNanostructure and molecular mechanics of spider dragline silk protein assemblies.
Spider silk is a self-assembling biopolymer that outperforms most known materials in terms of its mechanical performance, despite its underlying weak chemical bonding based on H-bonds. While experimental studies have shown that the molecular structure of silk proteins has a direct influence on the stiffness, toughness and failure strength of silk, no molecular-level analysis of the nanostructur...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)41777-2